辛夷标准汤剂质量标准研究(1)

http://www.100md.com 2020年3月1日 《中国中医药信息杂志》 20203

     摘要：目的 建立辛夷标准汤剂质量控制方法，为辛夷配方颗粒的质量评价提供依据。 方法 制备12批不同产地辛夷饮片的标准汤剂，按2015年版《中华人民共和国药典》挥发油测定法测定挥发油含量，采用超高效液相色谱(UPLC)-二极管阵列检测器对木兰脂素进行含量测定，计算出膏率和指标成分转移率，采用《中药色谱指纹图谱相似度评价系统(2004A)》软件对UPLC图谱进行分析。结果 12批辛夷饮片标准汤剂挥发油含量均大于1.0%(mL/g)，出膏率为14.0%～19.7%，木兰脂素转移率为6.61%～36.63%。UPLC图谱确定了4个共有峰，特征图谱相似度均大于0.9。结论 本研究制备的辛夷标准汤剂质量稳定，特征图谱相似度高，方法精密度、稳定性和重复性良好，可为辛夷配方颗粒的质量控制提供参考。

    关键词：辛夷;标准汤剂;木兰脂素;超高效液相色谱法;指纹图谱

    中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2020)03-0053-04
, http://www.100md.com
    DOI：10.3969/j.issn.1005-5304.201903289

Quality Study of Magnoliae Flos Standard Decoction

LIAO Pengfei1，2， WANG Yajie2， HAO Danli2， LIU Li2， ZHAO Qinghe2

1. Tibetan Duorui Pharmaceutical Company， Changdu 854000， China; 2. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China

    Abstract： Objective To establish the quality control method for Magnoliae Flos standard decoction， and to provide references for the quality control protocol of Magnoliae Flos dispensing granules. Methods Twelve batches of Magnoliae Flos standard decoction were prepared. The content of volatile oil was determined according to the methods in Chinese Pharmacopoeia (2015Ed). Ultra-high performance liquid chromatography (UPLC) - diode array detector was used to determine the content of magnolin， and the paste rate and transfer rate were calculated. UPLC fingerprint was analyzed by using Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2004A). Results The contents of volatile oil of 12 batches of Magnoliae Flos standard decoction were all more than 1.0% (mL/g)， the extraction rates were 14.0%–19.7%， and the transfer rates of magnolin were 6.61%?36.63%. Moreover， 4 common chromatographic peaks were determined based on their fingerprints. The similarity of 12 batches of samples was all higher than 0.9. Conclusion The quality of 12 batches of Magnoliae Flos standard decoction is stable， the fingerprint similarity is high， and the precision， stability and repeatability of the method are good， which can provide references for the quality control of Magnoliae Flos dispensing granules., http://www.100md.com(廖鹏飞 王亚杰 郝单丽 刘丽 赵庆贺)

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